Your code says: I think I want to sleep before animals want bad pizza
The sentence itself is done correctly, but the one thing that I noticed is that your start and stop codons are still in mRNA form, not DNA form like the rest of your code is.
Anthropology 101 Blog, very good correction for Emily.
Emily, Anthro 101 Blog was correct about the coding problem. You can’t just plug the start/stop codons into the DNA. You needed to put them into the RNA and then reverse transcribe them with the rest of the molecule. In addition to that, because of the way you inserted your start/stop codons into your code, you ended up with uracils (U) in your DNA. This should have been a warning sign to you that something was wrong.
Your decoder is human, and was able to figure out your mistake and still solve the sentence. The problem is, we are trying to duplicate the actual process of protein synthesis, where an enzyme will have to read your DNA molecule and produce a functional RNA molecule for translation. An enzyme wouldn't have known what to do with your code because it would have just stopped at the uracils (enzymes are DNA specific). Even if it had gotten past that, it would have translated the start/stop codons and they no longer would have been read as actual start/stop codons.
I've posted two decodes below. The first one is kind of what I just described, with the enzyme translating the entire strand and the start/stop codons disappearing. An translation enzyme wouldn't have bothered to read this RNA, because there was no start codon to tell it to "go". For our purposes, without a start codon, I just had to start decoding from the beginning.
The second codon is just snipping out your RNA sentence between the improperly places start/stop codons, translated into your sentence.
DNA: GGUACGAUGCAAGTGCAAGGGAACGTAACATTAGGGCGTTGAUAGGAGGAA RNA: CCAUGCUACGUUCACGUUCCCUUGCAUUGUAAUCCCGCAACUAUCCUCCUU Codons: AUG CUA CGU UCA CGU UCC CUU GCA UUG UAA UCC CGC AAC UAU CCU CCU U (start) Was learn and learn into is bad to an into twist anthropology of become become U
DNA (plus start/stop codons): AUG CAAGTGCAAGGGAACGTAACATTAGGGCGTTGA UAG RNA (without changing start/stop codons): AUG GUUCACGUUCCCUUGCAUUGUAAUCCCGCAACU UAG Codons: AUG GUU CAC GUU CCC UUG CAU UGU AAU CCC GCA ACU UAG (start) I think I want to sleep before animals want bad pizza. (stop)
Your code says: I think I want to sleep before animals want bad pizza
ReplyDeleteThe sentence itself is done correctly, but the one thing that I noticed is that your start and stop codons are still in mRNA form, not DNA form like the rest of your code is.
That is correct! And yes, I did make that mistake, thank you for your keen eye! :)
DeleteAnthropology 101 Blog, very good correction for Emily.
ReplyDeleteEmily, Anthro 101 Blog was correct about the coding problem. You can’t just plug the start/stop codons into the DNA. You needed to put them into the RNA and then reverse transcribe them with the rest of the molecule. In addition to that, because of the way you inserted your start/stop codons into your code, you ended up with uracils (U) in your DNA. This should have been a warning sign to you that something was wrong.
Your decoder is human, and was able to figure out your mistake and still solve the sentence. The problem is, we are trying to duplicate the actual process of protein synthesis, where an enzyme will have to read your DNA molecule and produce a functional RNA molecule for translation. An enzyme wouldn't have known what to do with your code because it would have just stopped at the uracils (enzymes are DNA specific). Even if it had gotten past that, it would have translated the start/stop codons and they no longer would have been read as actual start/stop codons.
I've posted two decodes below. The first one is kind of what I just described, with the enzyme translating the entire strand and the start/stop codons disappearing. An translation enzyme wouldn't have bothered to read this RNA, because there was no start codon to tell it to "go". For our purposes, without a start codon, I just had to start decoding from the beginning.
The second codon is just snipping out your RNA sentence between the improperly places start/stop codons, translated into your sentence.
DNA: GGUACGAUGCAAGTGCAAGGGAACGTAACATTAGGGCGTTGAUAGGAGGAA
RNA: CCAUGCUACGUUCACGUUCCCUUGCAUUGUAAUCCCGCAACUAUCCUCCUU
Codons: AUG CUA CGU UCA CGU UCC CUU GCA UUG UAA UCC CGC AAC UAU CCU CCU U
(start) Was learn and learn into is bad to an into twist anthropology of become become U
DNA (plus start/stop codons):
AUG CAAGTGCAAGGGAACGTAACATTAGGGCGTTGA UAG
RNA (without changing start/stop codons):
AUG GUUCACGUUCCCUUGCAUUGUAAUCCCGCAACU UAG
Codons: AUG GUU CAC GUU CCC UUG CAU UGU AAU CCC GCA ACU UAG
(start) I think I want to sleep before animals want bad pizza. (stop)